Journal: Chromosoma

Article Title: CTCF is essential for proper mitotic spindle structure and anaphase segregation.

doi: 10.1007/s00412-023-00810-w

Figure Lengend Snippet: Fig. 4 Mitotic spindle structure is perturbed in CTCF knock- downs. A Example images of mitosis in wild-type and CTCF knockdowns c13 and c21 with labeled microtubules (green, α-tubulin) and DNA (magenta, Hoechst). Mitotic spindles were classified as abnormal mitotic spindle if they were tri-/ tetrapolar spindles (yellow) or other abnormal (blue) if DNA was substantially behind the spindle pole or no division was observed. For all images, see Supplemental Figure 1. B Graph of the percentage of abnormal mitotic spindles (WT n = 0/48; CTCF knockdown c13 n = 12/42; CTCF knockdown c21 n = 12/57; CENP-E inhibitor GSK923295 72 h n = 11/17). C Box and whisker plot of average and individual measurement of DNA position relative to the spindle pole. Purple dots represent spindles where DNA is > 1 μm behind the spindle pole (WT 0%, c13 30%, c21 12%, GSK923295 29%). Error bar represents standard error. Statistical tests are Student’s t-tests, significance denoted by *p < 0.05, **p < 0.01, and ***p < 0.001, while no signifi- cance is denoted by ns. Scale bar is 10 μm

Article Snippet: Coverslips were placed on 50-μL drops of the primary antibody mixture consisting of rat anti-alpha tubulin at 1:100 dilution (Novus biological); mouse anti-lamin A/C at 1:100 dilution (Cell Signaling Technology); and PBS with 5% BSA.

Techniques: Labeling, Knockdown, Whisker Assay

Journal: Frontiers in Immunology

Article Title: miR-210 promotes the anti-inflammatory phenotype and M2 polarization in murine macrophages

doi: 10.3389/fimmu.2025.1633163

Figure Lengend Snippet: Effects of miR-210-KO in M0 macrophages. (A) Enrichr dot-plot representation for pathways enriched among differentially expressed genes in miR-210-KO versus WT M0 macrophages. The signed odds ratio (x-axis) indicates over-representation in up-regulated (positive) or down-regulated (negative) genes. (B) Western blot analysis of pro-IL1β levels in M0 macrophages, with α-tubulin used as a loading control. Densitometry analysis performed using TotalLab. Data are presented as the mean ± SEM, n = 3 mice per condition. Statistical significance was determined using Student’s t test, *p< 0.05. (C) ELISA quantification of IL-6, TNF-α and IL-1β in the supernatant of M0 macrophages. Data are presented as mean ± SEM, n = 3 for IL-6 and IL-1β and n=4 for TNF-α.

Article Snippet: The membranes were incubated overnight at 4°C with antibodies against p53 (Abcam, #ab90363, 1:1000), TGF-β (Abcam, #ab215715, 1:1000), GAPDH (Abcam, #ab37168, 1:1000), IL-1β (Abcam, ab9722, 1:1000), αTubulin (R&D Systems, MAB9344 1:1000), β-actin (Sigma-Aldrich, A2228), and MMP-9 antibody (Novus Biologicals, NBP1-57840), prepared in 5% BSA in TBST.

Techniques: Western Blot, Control, Enzyme-linked Immunosorbent Assay